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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of pig brain using 66112-1-Ig (same clone as 66112-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of rat brain using 66112-1-Ig (same clone as 66112-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of mouse brain using 66112-1-Ig (same clone as 66112-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

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Iduronate 2 sulfatase Monoclonal antibody, PBS Only

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66112-1-PBS
Clone No.1A3F9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66112-1-PBS targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Iduronate 2 sulfatase fusion protein Ag19095 相同性解析による交差性が予測される生物種
Full Name iduronate 2-sulfatase
Calculated molecular weight312aa,35 kDa; 550aa,62 kDa
Observed molecular weight 76 kDa, 55 kDa
GenBank accession numberBC006170
Gene Symbol IDS
Gene ID (NCBI) 3423
RRIDAB_2881511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22304
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).