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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI35 Recombinant antibody, PBS Only

IFI35 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240063E1

Cat no : 83160-4-PBS

Synonyms

interferon induced protein 35, IFP35, IFP 35, Ifi-35, Ifi 35

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFI35 Recombinant antibody (83160-4-RR)

    WB analysis using 83160-4-RR (same clone as 83160-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83160-4-RR (IFI35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83160-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IFI35 Recombinant antibody (83160-4-RR)

    IHC staining of human ovary cancer using 83160-4-RR (same clone as 83160-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83160-4-RR (IFI35 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83160-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IFI35 Recombinant antibody (83160-4-RR)

    IHC staining of human ovary cancer using 83160-4-RR (same clone as 83160-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83160-4-RR (IFI35 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83160-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83160-4-RR

    Affinity and Kinetic Characterization of 83160-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83160-4-RR against Human IFI35 were performed. The affinity constant is 1.11 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83160-4-PBS is IFI35 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFI35 fusion protein Ag34653 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 35
Calculated molecular weight 31 kDa
Observed molecular weight31-35 kDa
GenBank accession numberBC001356
Gene symbol IFI35
Gene ID (NCBI) 3430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.