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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI35 Recombinant antibody

IFI35 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240063E1

Cat no : 83160-4-RR

Synonyms

interferon induced protein 35, IFP35, IFP 35, Ifi-35, Ifi 35

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFI35 Recombinant antibody (83160-4-RR)

    WB analysis using 83160-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83160-4-RR (IFI35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IFI35 Recombinant antibody (83160-4-RR)

    IHC staining of human ovary cancer using 83160-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83160-4-RR (IFI35 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IFI35 Recombinant antibody (83160-4-RR)

    IHC staining of human ovary cancer using 83160-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83160-4-RR (IFI35 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83160-4-RR

    Affinity and Kinetic Characterization of 83160-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83160-4-RR against Human IFI35 were performed. The affinity constant is 1.11 nM.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, HepG2 cells, SW480 cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83160-4-RR is IFI35 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFI35 fusion protein Ag34653 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 35
Calculated molecular weight 31 kDa
Observed molecular weight31-35 kDa
GenBank accession numberBC001356
Gene symbol IFI35
Gene ID (NCBI) 3430
RRIDAB_3670854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.

Protocols

Product Specific Protocols
WB protocol for IFI35 antibody 83160-4-RRDownload protocol
IHC protocol for IFI35 antibody 83160-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols