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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated IFI35 Recombinant an (CL750-83160-4)

    WB analysis using CL750-83160-4

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83160-4 (IFI35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CoraLite® Plus 750-conjugated IFI35 Recombinant antibody

IFI35 Uni-rAbTM Recombinant Antibody for WB
Cat No. CL750-83160-4
Clone No.240063E1

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Synonyms

FLJ21753, Ifi 35, IFI35, IFP 35, IFP35, interferon induced protein 35

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, A549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83160-4 targets IFI35 in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFI35 fusion protein Ag34653 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 35
Calculated molecular weight 31 kDa
Observed molecular weight31-35 kDa
GenBank accession numberBC001356
Gene Symbol IFI35
Gene ID (NCBI) 3430
RRIDAB_3673774
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP80217
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 IFI35 antibody CL750-83160-4Download protocol
Standard Protocols
Click here to view our Standard Protocols