IFI44 Recombinant antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83424-1-PBS in a different storage buffer formulation.

1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83424-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.