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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI44 Recombinant antibody

IFI44 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240022F12

Cat no : 83424-1-RR

Synonyms

p44, MTAP44, Microtubule-associated protein 44, Interferon-induced protein 44, interferon induced protein 44

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFI44 Recombinant antibody (83424-1-RR)

    WB analysis using 83424-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using IFI44 Recombinant antibody (83424-1-RR)

    FC experiment of A549 using 83424-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83424-1-RR

    Affinity and Kinetic Characterization of 83424-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83424-1-RR is IFI44 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFI44 fusion protein Ag26121 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 44
Calculated molecular weight 444 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC022870
Gene symbol IFI44
Gene ID (NCBI) 10561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFI44 antibody 83424-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols