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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI44 Recombinant antibody

IFI44 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240022F12

Cat no : 83424-1-RR

Synonyms

p44, MTAP44, Microtubule-associated protein 44, Interferon-induced protein 44, interferon induced protein 44

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFI44 Recombinant antibody (83424-1-RR)

    WB analysis using 83424-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using IFI44 Recombinant antibody (83424-1-RR)

    FC experiment of A549 using 83424-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83424-1-RR

    Affinity and Kinetic Characterization of 83424-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83424-1-RR is IFI44 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFI44 fusion protein Ag26121 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 44
Calculated molecular weight 444 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC022870
Gene symbol IFI44
Gene ID (NCBI) 10561
RRIDAB_3671073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFI44 antibody 83424-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols