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- KD/KO Validated
IFIH1/MDA5 Polyclonal antibody
IFIH1/MDA5 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 21775-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 51 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
| IP | See 7 publications below |
| CoIP | See 1 publications below |
| RIP | See 3 publications below |
Product Information
21775-1-AP targets IFIH1/MDA5 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFIH1/MDA5 fusion protein Ag16467 相同性解析による交差性が予測される生物種 |
| Full Name | interferon induced with helicase C domain 1 |
| Calculated molecular weight | 1025 aa, 117 kDa |
| Observed molecular weight | 117-125 kDa |
| GenBank accession number | BC111750 |
| Gene symbol | IFIH1 |
| Gene ID (NCBI) | 64135 |
| RRID | AB_10734593 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| IHC protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| IF protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| IP protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Cell Stem Cell ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress. | ||
Nat Commun MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation. | ||
Nat Commun HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction. | ||
EMBO J CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH RASHMI (Verified Customer) (11-27-2024) | Used for WB, Highly recommended
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