Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-21775 targets IFIH1 in IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFIH1 fusion protein Ag16467 相同性解析による交差性が予測される生物種 |
| Full Name | interferon induced with helicase C domain 1 |
| Calculated molecular weight | 1025 aa, 117 kDa |
| Observed molecular weight | 117-125 kDa |
| GenBank accession number | BC111750 |
| Gene Symbol | IFIH1 |
| Gene ID (NCBI) | 64135 |
| RRID | AB_2883087 |
| Conjugate | CoraLite®488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 491 nm / 516 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYX4 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL488 IFIH1 antibody CL488-21775 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |