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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated IFITM2/3 Monoclonal antibody

IFITM2/3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E8D12

Cat no : CL555-66081

Synonyms

IFITM3, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, DSPA2b, 2E8D12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite®555-conjugated IFITM2/3 Monoclonal antibo (CL555-66081)

    IF Staining of U2OS using CL555-66081

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®555 IFITM2/3 antibody (CL555-66081, Clone: 2E8D12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated IFITM2/3 Monoclonal antibo (CL555-66081)

    FC experiment of HeLa using CL555-66081

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IFITM2/3 (CL555-66081, Clone:2E8D12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66081 targets IFITM2/3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFITM2/3 fusion protein Ag17863 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 3 (1-8U)
Observed molecular weight 15-20 kDa
GenBank accession numberBC070243
Gene symbol IFITM3
Gene ID (NCBI) 10410
RRIDAB_2919672
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Protocols

Product Specific Protocols
IF protocol for CL555 IFITM2/3 antibody CL555-66081Download protocol
FC protocol for CL555 IFITM2/3 antibody CL555-66081Download protocol
Standard Protocols
Click here to view our Standard Protocols