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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using IFITM2-Specific Monoclonal antibody (66137-1-Ig)

    WB analysis of HepG2 using 66137-1-Ig (same clone as 66137-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of recombinant protein using IFITM2-Specific Monoclonal antibody (66137-1-Ig)

    WB analysis of recombinant protein using 66137-1-Ig (same clone as 66137-1-PBS)

    IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (66137-1-Ig) at dilution of 1:10,000. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IFITM2-Specific Monoclonal antibody (66137-1-Ig)

    WB analysis of HepG2 using 66137-1-Ig (same clone as 66137-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IFITM2-Specific Monoclonal antibody (66137-1-Ig)

    WB analysis of MCF-7 using 66137-1-Ig (same clone as 66137-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig (IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66137-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

IFITM2-Specific Monoclonal antibody, PBS Only

IFITM2-Specific Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66137-1-PBS
Clone No.3D5F7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IFITM2, IFITM 2, DSPA2c, 3D5F7, 18D

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66137-1-PBS targets IFITM2-Specific in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 2 (1-8D)
Observed molecular weight 15 kDa
GenBank accession numberNM_006435
Gene Symbol IFITM2
Gene ID (NCBI) 10581
RRIDAB_2881536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01629
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.