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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated IFITM3 Polyclonal antibody

IFITM3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11714

Synonyms

IP15, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated IFITM3 Polyclonal antibody (CL594-11714)

    IF Staining of HeLa using CL594-11714

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 IFITM3 antibody (CL594-11714) at dilution of 1:200, CL488-phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated IFITM3 Polyclonal antibody (CL594-11714)

    IF Staining of HeLa using CL594-11714

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 IFITM3 antibody (CL594-11714) at dilution of 1:200, CL488-phalloidin (green).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated IFITM3 Polyclonal antibody (CL594-11714)

    FC experiment of HeLa using CL594-11714

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human IFITM3 (CL594-11714) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11714 targets IFITM3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM3 fusion protein Ag2285 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 3 (1-8U)
Calculated molecular weight 133 aa, 15 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006794
Gene symbol IFITM3
Gene ID (NCBI) 10410
RRIDAB_2919772
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Protocols

Product Specific Protocols
IF protocol for CL594 IFITM3 antibody CL594-11714Download protocol
FC protocol for CL594 IFITM3 antibody CL594-11714Download protocol
Standard Protocols
Click here to view our Standard Protocols