CoraLite® Plus 488-conjugated IFN-gamma Monoclonal antibody
IFN-gamma Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E10G7
Cat no : CL488-69007
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | PMA+ionomycin+protein transport inhibitors for 6h human PBMCs |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-69007 targets IFN-gamma in FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IFN-gamma fusion protein HZ-1301 相同性解析による交差性が予測される生物種 |
Full Name | IFN gamma |
Gene symbol | IFNG |
Gene ID (NCBI) | 3458 |
RRID | AB_2919565 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 IFN-gamma antibody CL488-69007 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |