CoraLite® Plus 488 Anti-Rat IFN-gamma Rabbit Recombinant Antibody
IFN-gamma Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6K20
Cat no : CL488-98001
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | ConA, PMA and ionomycin stimulated rat splenocytes |
| Positive FC detected in | ConA, PMA and ionomycin stimulated rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-98001 targets IFN-gamma in FC (Intra) applications and shows reactivity with Rat samples.
| Tested Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | interferon gamma |
| Calculated molecular weight | 18 kDa |
| GenBank accession number | NM_138880 |
| Gene symbol | IFN-gamma |
| Gene ID (NCBI) | 25712 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IFN-gamma antibody CL488-98001 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
