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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT140 Recombinant antibody, PBS Only

IFT140 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230231D5

Cat no : 82947-7-PBS

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFT140 Recombinant antibody (82947-7-RR)

    WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IFT140 Recombinant antibody (82947-7-RR)

    WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT140 Recombinant antibody (82947-7-RR)

    IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82947-7-PBS

    Affinity and Kinetic Characterization of 82947-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 82947-7-PBS is IFT140 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
GenBank accession numberBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.