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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT140 Recombinant antibody

IFT140 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230231D5

Cat no : 82947-7-RR

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFT140 Recombinant antibody (82947-7-RR)

    WB analysis of HeLa using 82947-7-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using IFT140 Recombinant antibody (82947-7-RR)

    WB analysis of HepG2 using 82947-7-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT140 Recombinant antibody (82947-7-RR)

    IF Staining of hTERT-RPE1 using 82947-7-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Affinity and Kinetic Characterization of 82947-7-RR

    Affinity and Kinetic Characterization of 82947-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82947-7-RR is IFT140 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
Observed molecular weight150-165 kDa
GenBank accession numberBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

Protocols

Product Specific Protocols
WB protocol for IFT140 antibody 82947-7-RRDownload protocol
IF protocol for IFT140 antibody 82947-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols