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CoraLite® Plus 488-conjugated IFT140 Recombinant antibody

IFT140 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-82947-7
Clone No.230231D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -


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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82947-7 targets IFT140 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
Observed molecular weight150-165 kDa
GenBank accession numberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
RRIDAB_3673148
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RY7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IFT140 antibody CL488-82947-7Download protocol
Standard Protocols
Click here to view our Standard Protocols
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