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IFT81 Polyclonal antibody

Name: IFT81 Polyclonal antibody
Brand: Proteintech
SKU: 11744-1-AP

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11744-1-AP

Synonyms

CDV1R, CDV-1, CDV1, CDV 1R, CDV 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11744-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11744-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11744-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11744-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11744-1-AP
IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug.
IP experiment of mouse brain using 11744-1-AP
IP result of anti-IFT81 (IP:11744-1-AP, 4ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 5200ug.
IHC staining of human prostate cancer using 11744-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of hTERT-RPE1 cells using 11744-1-AP
IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim.
IF Staining of MDCK using 11744-1-AP
Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) for IFT81, and Rhodamine-Goat anti-rabbit IgG for 66200-1-Ig.
IF Staining of hTERT-RPE1 cells using 11744-1-AP
IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.
IF Staining of hTERT-RPE1 using 11744-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).
IF Staining of MDCK using 11744-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).
IF Staining of C2C12 using 11744-1-AP
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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Cat No. 11744-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, mouse testis tissue, HEK-293 cells, human brain tissue, rat testis tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	C2C12 cells, hTERT-RPE1 cells, MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 24 publications below
IHC	See 1 publications below
IF	See 32 publications below
CoIP	See 1 publications below

Product Information

11744-1-AP targets IFT81 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IFT81 fusion protein Ag2339 相同性解析による交差性が予測される生物種
Full Name	intraflagellar transport 81 homolog (Chlamydomonas)
Calculated molecular weight	676 aa, 80 kDa
Observed molecular weight	75-80 kDa
GenBank accession number	BC029349
Gene symbol	IFT81
Gene ID (NCBI)	28981
RRID	AB_2121966
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IFT81 antibody 11744-1-AP	Download protocol
IHC protocol for IFT81 antibody 11744-1-AP	Download protocol
IF protocol for IFT81 antibody 11744-1-AP	Download protocol
IP protocol for IFT81 antibody 11744-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. Authors - Siew Cheng Phua View Article
mouse	IF	Nat Cell Biol The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment. Authors - Mu He View Article
human	WB	Nat Cell Biol Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Authors - Malavika Raman View Article
human	WB	Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. Authors - Nicolas Taulet View Article
mouse	WB	Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. Authors - Kai Hao View Article
mouse	WB,IF	EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. Authors - Shichao Duan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11744-1-AP

WB analysis of mouse testis using 11744-1-AP

WB analysis of HEK-293 using 11744-1-AP

WB analysis of human brain using 11744-1-AP

IP experiment of mouse brain using 11744-1-AP

IP experiment of mouse brain using 11744-1-AP

IHC staining of human prostate cancer using 11744-1-AP

IF Staining of hTERT-RPE1 cells using 11744-1-AP

IF Staining of MDCK using 11744-1-AP

IF Staining of hTERT-RPE1 cells using 11744-1-AP

IF Staining of hTERT-RPE1 using 11744-1-AP

IF Staining of MDCK using 11744-1-AP

IF Staining of C2C12 using 11744-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment.

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.