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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11744

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated IFT81 Polyclonal ant (CL488-11744)

    IF Staining of hTERT-RPE1 using CL488-11744

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated IFT81 Polyclonal ant (CL488-11744)

    IF Staining of MDCK using CL488-11744

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated IFT81 Polyclonal ant (CL488-11744)

    IF Staining of MDCK using CL488-11744

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:50, CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11744 targets IFT81 in IF/ICC applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT81 fusion protein Ag2339 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Calculated molecular weight 676 aa, 80 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC029349
Gene symbol IFT81
Gene ID (NCBI) 28981
RRIDAB_2919039
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IFT81 antibody CL488-11744Download protocol
Standard Protocols
Click here to view our Standard Protocols