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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFT81 Monoclonal antibody (67987-1-Ig)

    WB analysis using 67987-1-Ig (same clone as 67987-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using IFT81 Monoclonal antibody (67987-1-Ig)

    WB analysis of rabbit brain using 67987-1-Ig (same clone as 67987-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFT81 Monoclonal antibody (67987-1-Ig)

    IHC staining of mouse testis using 67987-1-Ig (same clone as 67987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFT81 Monoclonal antibody (67987-1-Ig)

    IHC staining of mouse testis using 67987-1-Ig (same clone as 67987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67987-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT81 Monoclonal antibody (67987-1-Ig)

    IF Staining of hTERT-RPE1 using 67987-1-Ig (same clone as 67987-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (67987-1-Ig, Clone: 1D5A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 67987-1-PBS in a different storage buffer formulation.

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IFT81 Monoclonal antibody, PBS Only

IFT81 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67987-1-PBS
Clone No.1D5A3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Rabbit, canine

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67987-1-PBS targets IFT81 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, canine samples.

Tested Reactivity Human, Rat, Mouse, Rabbit, canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFT81 fusion protein Ag31629 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Calculated molecular weight 676 aa, 80 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC029349
Gene Symbol IFT81
Gene ID (NCBI) 28981
RRIDAB_2918736
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WYA0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.