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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGF2BP2 Recombinant antibody (82757-2-RR)

    WB analysis using 82757-2-RR (same clone as 82757-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82757-2-RR (IGF2BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using IGF2BP2 Recombinant antibody (82757-2-RR)

    IP experiment of HEK-293 using 82757-2-RR (same clone as 82757-2-PBS)

    IP result of anti-IGF2BP2 (IP:82757-2-RR, 4ug; Detection:82757-2-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82757-2-RR

    Affinity and Kinetic Characterization of 82757-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82757-2-RR against Human IGF2BP2 were performed. The affinity constant is below 1 pM.

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IGF2BP2 Recombinant antibody, PBS Only

IGF2BP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82757-2-PBS
Clone No.6L8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82757-2-PBS targets IGF2BP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IGF2BP2 fusion protein Ag2142 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 2 mRNA binding protein 2
Calculated molecular weight65 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC021290
Gene Symbol IGF2BP2
Gene ID (NCBI) 10644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6M1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).