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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGF2BP2 Recombinant antibody

IGF2BP2 Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

6L8

Cat no : 82757-2-RR

Synonyms

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGF2BP2 Recombinant antibody (82757-2-RR)

    WB analysis using 82757-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82757-2-RR (IGF2BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using IGF2BP2 Recombinant antibody (82757-2-RR)

    IP experiment of HEK-293 using 82757-2-RR

    IP result of anti-IGF2BP2 (IP:82757-2-RR, 4ug; Detection:82757-2-RR 1:4000) with HEK-293 cells lysate 1480 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of mouse kidney using 82757-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of mouse kidney using 82757-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of human pancreas cancer using 82757-2-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP2 Recombinant antibody (82757-2-RR)

    IHC staining of human pancreas cancer using 82757-2-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82757-2-RR

    Affinity and Kinetic Characterization of 82757-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82757-2-RR against Human IGF2BP2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82757-2-RR targets IGF2BP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IGF2BP2 fusion protein Ag2142 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 2 mRNA binding protein 2
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC021290
Gene symbol IGF2BP2
Gene ID (NCBI) 10644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

Protocols

Product Specific Protocols
WB protocol for IGF2BP2 antibody 82757-2-RRDownload protocol
IHC protocol for IGF2BP2 antibody 82757-2-RRDownload protocol
IP protocol for IGF2BP2 antibody 82757-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols