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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody

IGF2BP3 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1L11

Cat no : CL750-81805

Synonyms

DKFZp686F1078, hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated IGF2BP3 Recombinant (CL750-81805)

    WB analysis using CL750-81805

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated IGF2BP3 Recombinant (CL750-81805)

    WB analysis using CL750-81805

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81805 (IGF2BP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNIH/3T3 cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81805 targets IGF2BP3 in WB applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IGF2BP3 fusion protein Ag6226 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight 64 kDa
Observed molecular weight64 kDa
GenBank accession numberBC065269
Gene symbol IGF2BP3
Gene ID (NCBI) 10643
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 IGF2BP3 antibody CL750-81805Download protocol
Standard Protocols
Click here to view our Standard Protocols