CoraLite® Plus 750-conjugated IGF2BP3 Recombinant antibody
IGF2BP3 Recombinant Antibody for WB, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
1L11
Cat no : CL750-81805
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HepG2 cells, K-562 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-81805 targets IGF2BP3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IGF2BP3 fusion protein Ag6226 相同性解析による交差性が予測される生物種 |
| Full Name | insulin-like growth factor 2 mRNA binding protein 3 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC065269 |
| Gene symbol | IGF2BP3 |
| Gene ID (NCBI) | 10643 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 IGF2BP3 antibody CL750-81805 | Download protocol |
| FC protocol for CL Plus 750 IGF2BP3 antibody CL750-81805 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
