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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using IGFBP1 Recombinant antibody (83347-5-RR)

    WB analysis of HepG2 using 83347-5-RR (same clone as 83347-5-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83347-5-RR (IGFBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83347-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83347-5-RR

    Affinity and Kinetic Characterization of 83347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83347-5-RR against Human IGFBP1 were performed. The affinity constant is 40.6 nM.

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IGFBP1 Recombinant antibody, PBS Only

IGFBP1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83347-5-PBS
Clone No.240263B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83347-5-PBS targets IGFBP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 1
Calculated molecular weight28 kDa
GenBank accession numberNM_000596.4
Gene Symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08833
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.