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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP1 Recombinant antibody

IGFBP1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240263B9

Cat no : 83347-5-RR

Synonyms

IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using IGFBP1 Recombinant antibody (83347-5-RR)

    WB analysis of HepG2 using 83347-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83347-5-RR (IGFBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83347-5-RR

    Affinity and Kinetic Characterization of 83347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83347-5-RR against Human IGFBP1 were performed. The affinity constant is 40.6 nM.

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Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83347-5-RR is IGFBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 1
Calculated molecular weight28 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberNM_000596.4
Gene symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

Protocols

Product Specific Protocols
WB protocol for IGFBP1 antibody 83347-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols