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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IGFBP2 Monoclonal antibody

IGFBP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F10D1

Cat no : CL488-66644

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated IGFBP2 Monoclonal an (CL488-66644)

    IF Staining of MCF-7 using CL488-66644

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 IGFBP2 antibody (CL488-66644, Clone: 2F10D1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated IGFBP2 Monoclonal an (CL488-66644)

    FC experiment of MCF-7 using CL488-66644

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IGFBP2 (CL488-66644, Clone:2F10D1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66644 targets IGFBP2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGFBP2 fusion protein Ag8421 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004312
Gene symbol IGFBP2
Gene ID (NCBI) 3485
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IGFBP2 antibody CL488-66644Download protocol
FC protocol for CL Plus 488 IGFBP2 antibody CL488-66644Download protocol
Standard Protocols
Click here to view our Standard Protocols