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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP5 Monoclonal antibody

IGFBP5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5E1

Cat no : 68512-1-Ig

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP5 Monoclonal antibody (68512-1-Ig)

    WB analysis using 68512-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68512-1-Ig (IGFBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IGFBP5 Monoclonal antibody (68512-1-Ig)

    IHC staining of mouse brain using 68512-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68512-1-Ig (IGFBP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IGFBP5 Monoclonal antibody (68512-1-Ig)

    IHC staining of mouse brain using 68512-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68512-1-Ig (IGFBP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inT-47D cells, SKOV-3 cells, Saos-2 cells, U2OS cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68512-1-Ig targets IGFBP5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGFBP5 fusion protein Ag33797 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 5
Calculated molecular weight 31 kDa
Observed molecular weight30-33 kDa
GenBank accession numberBC011453
Gene symbol IGFBP5
Gene ID (NCBI) 3488
RRIDAB_3085221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Protocols

Product Specific Protocols
WB protocol for IGFBP5 antibody 68512-1-IgDownload protocol
IHC protocol for IGFBP5 antibody 68512-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols