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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP5 Monoclonal antibody (68512-1-Ig)

    WB analysis using 68512-1-Ig (same clone as 68512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68512-1-Ig (IGFBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IGFBP5 Monoclonal antibody (68512-1-Ig)

    IHC staining of mouse brain using 68512-1-Ig (same clone as 68512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68512-1-Ig (IGFBP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IGFBP5 Monoclonal antibody (68512-1-Ig)

    IHC staining of mouse brain using 68512-1-Ig (same clone as 68512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68512-1-Ig (IGFBP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68512-1-PBS in a different storage buffer formulation.

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IGFBP5 Monoclonal antibody, PBS Only

IGFBP5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68512-1-PBS
Clone No.1C5E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68512-1-PBS targets IGFBP5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGFBP5 fusion protein Ag33797 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 5
Calculated molecular weight 31 kDa
Observed molecular weight30-33 kDa
GenBank accession numberBC011453
Gene Symbol IGFBP5
Gene ID (NCBI) 3488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24593
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.