Human IgA Heavy Chain Polyclonal antibody
Human IgA Heavy Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 11449-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue, human lung tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive FC (Intra) detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
11449-1-AP targets Human IgA Heavy Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human IgA Heavy Chain fusion protein Ag2007 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant alpha 1 |
| Calculated molecular weight | 496 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC016369 |
| Gene symbol | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_833092 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
| IHC protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
| IF protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Infect Immune responses and residual SARS-CoV-2 in two critically ill COVID-19 patients before and after lung transplantation. | ||
Cell Immunol Mesenchymal stem cell transplantation worsens intestinal inflammation and microenvironment in PI3Kγ-knockout mice | ||
Sci Rep A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer | ||
Int Immunopharmacol Altered microbial community structure in PI3Kγ knockout mice with colitis impeding relief of inflammation: Establishment of new indices for intestinal microbial disorder. | ||
Evid Based Complement Alternat Med Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress. |
