Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-11449 targets Human IgA Heavy Chain in IHC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human IgA Heavy Chain fusion protein Ag2007 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant alpha 1 |
| Calculated molecular weight | 496 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC016369 |
| Gene Symbol | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_2918936 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin Human IgA Heavy Chain antibody Biotin-11449 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |