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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin-conjugated human IgD Monoclonal antibody (Biotin-67538)

    IHC staining of human tonsillitis using Biotin-67538

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-67538 (human IgD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Biotin-conjugated IgD Monoclonal antibody

human IgD Monoclonal Antibody for IHC
Cat No. Biotin-67538
Clone No.1D1B12

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC

Synonyms

FLJ00382, FLJ46727, IGHD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-67538 targets human IgD in IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen human IgD fusion protein Ag9614 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2918950
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .

Protocols

Product Specific Protocols
IHC protocol for Biotin human IgD antibody Biotin-67538Download protocol
Standard Protocols
Click here to view our Standard Protocols