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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IKKA Recombinant antibody (84372-4-RR)

    WB analysis using 84372-4-RR (same clone as 84372-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84372-4-RR (IKKA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84372-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using IKKA Recombinant antibody (84372-4-RR)

    FC experiment of HepG2 using 84372-4-RR (same clone as 84372-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant antibody (84372-4-RR, Clone:241579A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84372-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84372-4-RR

    Affinity and Kinetic Characterization of 84372-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84372-4-RR against Human IKKA were performed. The affinity constant is 19.5 nM.

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IKKA Recombinant antibody, PBS Only

IKKA Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84372-4-PBS
Clone No.241579A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84372-4-PBS targets IKKA in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IKKA fusion protein Ag26573 相同性解析による交差性が予測される生物種
Full Name conserved helix-loop-helix ubiquitous kinase
Calculated molecular weight 85 kDa
Observed molecular weight85 kDa
GenBank accession numberNM_001278
Gene Symbol IKK Alpha
Gene ID (NCBI) 1147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15111
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.