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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IKKA Recombinant antibody

IKKA Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241579A2

Cat no : 84372-4-RR

Synonyms

CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IKKA Recombinant antibody (84372-4-RR)

    WB analysis using 84372-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84372-4-RR (IKKA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using IKKA Recombinant antibody (84372-4-RR)

    FC experiment of HepG2 using 84372-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant antibody (84372-4-RR, Clone:241579A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84372-4-RR

    Affinity and Kinetic Characterization of 84372-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84372-4-RR against Human IKKA were performed. The affinity constant is 19.5 nM.

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Tested Applications

Positive WB detected inJurkat cells, Daudi cells, HeLa cells, HepG2 cells, RAW 264.7 cells, C6 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84372-4-RR is IKKA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IKKA fusion protein Ag26573 相同性解析による交差性が予測される生物種
Full Name conserved helix-loop-helix ubiquitous kinase
Calculated molecular weight 85 kDa
Observed molecular weight85 kDa
GenBank accession numberNM_001278
Gene symbol IKK Alpha
Gene ID (NCBI) 1147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IKKA antibody 84372-4-RRDownload protocol
FC protocol for IKKA antibody 84372-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols