IKZF1 Monoclonal antibody
IKZF1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, Rabbit, Pig and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D6B5
Cat no : 66966-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Jurkat cells, pig thymus tissue, rabbit spleen tissue, Ramos cells, Daudi cells, Sp2/0 cells, MOLT-4 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66966-1-Ig targets IKZF1 in WB, ELISA applications and shows reactivity with Human, mouse, Rabbit, Pig samples.
| Tested Reactivity | Human, mouse, Rabbit, Pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IKZF1 fusion protein Ag28637 相同性解析による交差性が予測される生物種 |
| Full Name | IKAROS family zinc finger 1 (Ikaros) |
| Calculated molecular weight | 477 aa, 53 kDa |
| Observed molecular weight | 55-65 kDa |
| GenBank accession number | BC018349 |
| Gene symbol | IKZF1 |
| Gene ID (NCBI) | 10320 |
| RRID | AB_2882289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IKZF1 antibody 66966-1-Ig | Download protocol |
| IHC protocol for IKZF1 antibody 66966-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2. |
