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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IKZF1 Monoclonal antibody, PBS Only

IKZF1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, Rabbit, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66966-1-PBS

Synonyms

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IKZF1 Monoclonal antibody (66966-1-Ig)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit spleen tissue using IKZF1 Monoclonal antibody (66966-1-Ig)

    WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig thymus tissue using IKZF1 Monoclonal antibody (66966-1-Ig)

    WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IKZF1 Monoclonal antibody (66966-1-Ig)

    WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using IKZF1 Monoclonal antibody (66966-1-Ig)

    IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66966-1-PBS targets IKZF1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, Rabbit, Pig samples.

Tested Reactivity Human, mouse, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IKZF1 fusion protein Ag28637 相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 1 (Ikaros)
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC018349
Gene symbol IKZF1
Gene ID (NCBI) 10320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.