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  • Featured Product
  • KD/KO Validated

IL-1 Beta Monoclonal antibody, PBS Only (Detector)

IL-1 Beta Monoclonal Antibody for WB, FC, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1B4

Cat no : 66737-1-PBS

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    WB analysis of A431 using 66737-1-Ig (same clone as 66737-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    WB analysis of U-87 MG using 66737-1-Ig (same clone as 66737-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP80001-6

    Sandwich ELISA standard curve of MP80001-6

    Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-RR. Detection antibody: HRP-conjugated 66737-1-PBS. Standard:HZ-1164. Range: 0.098-12.5 ng/mL.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using IL-1 Beta Monoclonal antibody (66737-1-Ig)

    FC experiment of THP-1 using 66737-1-Ig (same clone as 66737-1-PBS)

    1X10^6 THP-1 cells untreated (dashed lines), LPS treated (red) were intracellularly stained with 0.4 ug Anti-Human IL-1 Beta (66737-1-Ig, Clone:2A1B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66737-1-PBS targets IL-1 Beta as part of a matched antibody pair:

MP80001-6: 82696-8-PBS capture and 66737-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 Beta fusion protein Ag26030 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.