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KD/KO Validated

IL-1 Beta Monoclonal antibody

Name: IL-1 Beta Monoclonal antibody
Brand: Proteintech
SKU: 66737-1-Ig

IL-1 Beta Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF, IP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

CloneNo.

2A1B4

Review Product

Cat no : 66737-1-Ig

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Validation Data Gallery

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AllWBIHC

WB analysis of A431 using 66737-1-Ig
A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of U-87 MG using 66737-1-Ig
U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 66737-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 66737-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 66737-1-Ig
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin cancer using 66737-1-Ig
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human skin cancer using 66737-1-Ig
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human skin cancer using 66737-1-Ig
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens).

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Cat No. 66737-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, U-87 MG cells
Positive IHC detected in	human lung cancer tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 65 publications below
IHC	See 12 publications below
IF	See 7 publications below
IP	See 1 publications below
ELISA	See 2 publications below

Product Information

66737-1-Ig targets IL-1 Beta in WB, IHC, IF, IP, ELISA, Cell treatment applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human, rabbit, bovine
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	IL-1 Beta fusion protein Ag26030 相同性解析による交差性が予測される生物種
Full Name	interleukin 1, beta
Calculated molecular weight	269 aa, 31 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC008678
Gene symbol	IL1B
Gene ID (NCBI)	3553
RRID	AB_2882087
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Protocols

Product Specific Protocols
WB protocol for IL-1 Beta antibody 66737-1-Ig	Download protocol
IHC protocol for IL-1 Beta antibody 66737-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Biomaterials Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo. Authors - Angelina D Schoenenberger View Article
	IHC	J Nanobiotechnology Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression Authors - Yunhao Wang View Article
	WB,IHC	Cell Mol Life Sci circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells Authors - Yang Bai View Article
	WB	Oxid Med Cell Longev Ellagic Acid Protects Dopamine Neurons via Inhibition of NLRP3 Inflammasome Activation in Microglia. Authors - Xue-Mei He View Article
	WB	Arch Toxicol SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice Authors - Jia-Wen Chen View Article
	WB	Aging (Albany NY) Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis. Authors - Zhenglan Han View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1 Beta Monoclonal antibody

IL-1 Beta Monoclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of A431 using 66737-1-Ig

WB analysis of U-87 MG using 66737-1-Ig

IHC staining of human lung cancer using 66737-1-Ig

IHC staining of human lung cancer using 66737-1-Ig

IHC staining of human skin cancer using 66737-1-Ig

IHC staining of human skin cancer using 66737-1-Ig

IHC staining of human skin cancer using 66737-1-Ig

IHC staining of human skin cancer using 66737-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.

Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression

circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells

Ellagic Acid Protects Dopamine Neurons via Inhibition of NLRP3 Inflammasome Activation in Microglia.

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice

Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis.