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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis using 13356-1-AP (same clone as 13356-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL-10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis of human liver using 13356-1-AP (same clone as 13356-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis of human heart using 13356-1-AP (same clone as 13356-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis of human placenta using 13356-1-AP (same clone as 13356-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13356-1-PBS targets IL-10RA in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10RA fusion protein Ag4156 相同性解析による交差性が予測される生物種
Full Name interleukin 10 receptor, alpha
Calculated molecular weight 578 aa, 63 kDa
Observed molecular weight 70-72 kDa, 90-95 kDa
GenBank accession numberBC028082
Gene Symbol IL-10RA
Gene ID (NCBI) 3587
RRIDAB_10643390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13651
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356).