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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-12 p70 Recombinant antibody

IL-12 p70 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230295F4

Cat no : 83094-1-RR

Synonyms

IL-12, Interleukin 12, IL12, IL 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-12 Recombinant antibody (83094-1-RR)

    WB analysis using 83094-1-RR

    Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IL-12 p70 Recombinant antibody (83094-1-RR)

    WB analysis using 83094-1-RR

    Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83094-1-RR

    Affinity and Kinetic Characterization of 83094-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83094-1-RR against Human IL-12 p70 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83094-1-RR targets IL-12 p70 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name IL-12 p70
Observed molecular weight70 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_3670808
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.

Protocols

Product Specific Protocols
WB protocol for IL-12 p70 antibody 83094-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols