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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-12 p70 Recombinant antibody (83094-1-RR)

    WB analysis using 83094-1-RR (same clone as 83094-1-PBS)

    Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IL-12 p70 Recombinant antibody (83094-1-RR)

    WB analysis using 83094-1-RR (same clone as 83094-1-PBS)

    Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83094-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83094-1-RR

    Affinity and Kinetic Characterization of 83094-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83094-1-RR against Human IL-12 p70 were performed. The affinity constant is below 1 pM.

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IL-12 p70 Recombinant antibody, PBS Only

IL-12 p70 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83094-1-PBS
Clone No.230295F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IL-12, Interleukin 12, IL12, IL 12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83094-1-PBS targets IL-12 p70 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name IL-12 p70
Observed molecular weight70 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29460(IL12B);P29459(IL12A)
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.