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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human IL-12 (1E12H6)

IL-12 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

1E12H6

Cat no : FITC-65277

Synonyms

IL-12, IL-12 p35, IL-12 subunit p35, IL12A, IL-12A, Interleukin 12 subunit alpha, Interleukin-12 subunit alpha, NFSK, NKSF1, P35, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human IL-12 (1E12H6) (FITC-65277)

    FC experiment of human PBMCs using FITC-65277

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-12 (FITC-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human IL-12 (1E12H6) (FITC-65277)

    FC experiment of human PBMCs using FITC-65277

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-12 (FITC-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-12 (FITC-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65277 targets IL-12 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-12 fusion protein HZ-1256 相同性解析による交差性が予測される生物種
Full Name interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Calculated molecular weight 25 kDa
GenBank accession numberBC104982
Gene symbol IL-12A
Gene ID (NCBI) 3592
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

Protocols

Product Specific Protocols
FC protocol for FITC Plus IL-12 antibody FITC-65277Download protocol
Standard Protocols
Click here to view our Standard Protocols