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Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using PE Anti-Mouse IL-12/IL-23 p40 (C17.8) (PE-65097)

    FC experiment of RAW 264.7 using PE-65097

    1X10^6 untreated or IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells were intracellularly stained with 0.5 ug PE Anti-Mouse IL-12/IL-23 p40 (C17.8) (PE-65097, Clone: C17.8) (red), or 0.5 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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PE Anti-Mouse IL-12/IL-23 p40 (C17.8)

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)
Cat No. PE-65097
Clone No.C17.8

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inIFNγ and LPS treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen CHO expressed, recombinant mouse IL-12 p70 相同性解析による交差性が予測される生物種
Full Name interleukin 12b
GenBank accession numberBC103608
Gene Symbol Il12b
Gene ID (NCBI) 16160
RRIDAB_2920518
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP43432
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Protocols

Product Specific Protocols
FC protocol for PE IL-12/IL-23 p40 antibody PE-65097Download protocol
Standard Protocols
Click here to view our Standard Protocols