- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated IL-12RB1 Monoclonal antibody
IL-12RB1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E10E10
Cat no : CL488-66829
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66829 targets IL-12RB1 in FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-12RB1 fusion protein Ag6920 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 12 receptor, beta 1 |
Calculated molecular weight | 662 aa, 73 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC029121 |
Gene symbol | IL12RB1 |
Gene ID (NCBI) | 3594 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Protocols
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 IL-12RB1 antibody CL488-66829 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |