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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-21R Polyclonal antibody, PBS Only

IL-21R Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10533-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IL-21R Polyclonal antibody (10533-1-AP)

    WB analysis using 10533-1-AP (same clone as 10533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using IL-21R Polyclonal antibody (10533-1-AP)

    WB analysis of K-562 using 10533-1-AP (same clone as 10533-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using IL-21R Polyclonal antibody (10533-1-AP)

    WB analysis of mouse thymus using 10533-1-AP (same clone as 10533-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using IL-21R Polyclonal antibody (10533-1-AP)

    WB analysis of Raji using 10533-1-AP (same clone as 10533-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using IL-21R Polyclonal antibody (10533-1-AP)

    WB analysis of COLO 320 using 10533-1-AP (same clone as 10533-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using IL-21R Polyclonal antibody (10533-1-AP)

    IHC staining of human lymphoma using 10533-1-AP (same clone as 10533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using IL-21R Polyclonal antibody (10533-1-AP)

    IHC staining of human lymphoma using 10533-1-AP (same clone as 10533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using IL-21R Polyclonal antibody (10533-1-AP)

    IF Staining of COLO 320 using 10533-1-AP (same clone as 10533-1-PBS)

    Immunofluorescent analysis of COLO 320 cells using 10533-1-AP (IL21R antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10533-1-PBS targets IL-21R in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-21R fusion protein Ag0762 相同性解析による交差性が予測される生物種
Full Name interleukin 21 receptor
Calculated molecular weight 59 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC004348
Gene symbol IL-21R
Gene ID (NCBI) 50615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.