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  • Featured Product
  • KD/KO Validated

IL-4 Monoclonal antibody, PBS Only

IL-4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G2C5

Cat no : 66142-1-PBS

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra, PS1TP5BP1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IL-4 Monoclonal antibody (66142-1-Ig)

    WB analysis of Jurkat using 66142-1-Ig (same clone as 66142-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig (IL-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-4 Monoclonal antibody (66142-1-Ig)

    IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-4 Monoclonal antibody (66142-1-Ig)

    IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using IL-4 Monoclonal antibody (66142-1-Ig)

    IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using IL-4 Monoclonal antibody (66142-1-Ig)

    IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Ramos cells using IL-4 Monoclonal antibody (66142-1-Ig)

    FC experiment of Ramos using 66142-1-Ig (same clone as 66142-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human IL-4 (66142-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66142-1-PBS targets IL-4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-4 fusion protein Ag9940 相同性解析による交差性が予測される生物種
Full Name interleukin 4
Calculated molecular weight 153 aa, 17 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC070123
Gene symbol IL-4
Gene ID (NCBI) 3565
RRIDAB_2881539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.