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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-6 Monoclonal antibody, PBS Only (Detector)

IL-6 Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

6C4B7

Cat no : 68659-2-PBS

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

Validation Data Gallery

Filter:
  • Sandwich ELISA standard curve of MP50032-1

    Sandwich ELISA standard curve of MP50032-1

    Sandwich ELISA standard curve of MP50032-1, IL6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. Standard: HZ-1019. Range: 7.8-500 pg/mL.

  • Sample test of MP50032-1

    Sample test of MP50032-1

    Cytometric bead array of HT-1080 cells supernatant using MP50032-1, IL-6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. HT-1080 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 6 days in normal culture media or IL-17A (Cat.NO. HZ-1113, 40 ng/mL) containing media. 20 μL of the supernatant from day 1, 2, 3 and day 6 was analyzed using MP50032-1 (IL-6 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time.Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array standard curve of MP50032-1

    Cytometric bead array standard curve of MP50032-1

    Cytometric bead array standard curve of MP50032-1, IL-6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. Standard:HZ-1019. Range: 0.098-12.5 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68659-2-PBS targets IL-6 as part of a matched antibody pair:

MP50032-1: 68659-1-PBS capture and 68659-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-6 fusion protein HZ-1019 相同性解析による交差性が予測される生物種
Full Name interleukin 6 (interferon, beta 2)
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.