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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody

IL-7Ra/CD127 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240749H2

Cat no : CL488-98074

Synonyms

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit (CL488-98074)

    FC experiment of human PBMCs using CL488-98074

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (CL488-98074, Clone: 240749H2) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit (CL488-98074)

    FC experiment of human PBMCs using CL488-98074

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (CL488-98074, Clone: 240749H2) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98074 targets IL-7Ra/CD127 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 7 receptor
Calculated molecular weight52 kDa
GenBank accession numberAAC83204.1
Gene symbol CD127
Gene ID (NCBI) 3575
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IL-7Ra/CD127 antibody CL488-98074Download protocol
Standard Protocols
Click here to view our Standard Protocols