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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL12RB1 Recombinant antibody, PBS Only (Detector)

IL12RB1 Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241661D7

Cat no : 84391-4-PBS

Synonyms

IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1, CD212

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using IL12RB1 Recombinant antibody (84391-4-RR)

    IF Staining of K-562 using 84391-4-RR (same clone as 84391-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01262-2

    Cytometric bead array standard curve of MP01262-2

    Cytometric bead array standard curve of MP01262-2, IL12RB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84391-3-PBS. Detection antibody: 84391-4-PBS. Standard: RP00005. Range: 0.781-50 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84391-4-PBS targets IL12RB1 as part of a matched antibody pair:

MP01262-2: 84391-3-PBS capture and 84391-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 12 receptor, beta 1
Calculated molecular weight 662 aa, 73 kDa
GenBank accession numberBC029121
Gene symbol IL12RB1
Gene ID (NCBI) 3594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.