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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL12RB1 Recombinant antibody

IL12RB1 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241661D7

Cat no : 84391-4-RR

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using IL12RB1 Recombinant antibody (84391-4-RR)

    IF Staining of K-562 using 84391-4-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84391-4-RR targets IL12RB1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 12 receptor, beta 1
Calculated molecular weight 662 aa, 73 kDa
GenBank accession numberBC029121
Gene symbol IL12RB1
Gene ID (NCBI) 3594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Protocols

Product Specific Protocols
IF protocol for IL12RB1 antibody 84391-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols