IL-2 Monoclonal antibody

IL-2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F11C5

Cat no : 60306-1-Ig

Synonyms

IL2, TCGF, Interleukin-2, interleukin 2, IL 2


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Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60306-1-Ig targets IL-2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-2 fusion protein Ag10199 相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight 153 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC070338
Gene symbol IL2
Gene ID (NCBI) 3558
RRIDAB_2881420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
WB protocol for IL-2 antibody 60306-1-IgDownload protocol
IHC protocol for IL-2 antibody 60306-1-IgDownload protocol
IF protocol for IL-2 antibody 60306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo
mouseWB

PLoS One

N-Acetylcysteine Attenuates Ischemia-Reperfusion-Induced Apoptosis and Autophagy in Mouse Liver via Regulation of the ROS/JNK/Bcl-2 Pathway.

Authors - Chengfen Wang
mouseWB

Inflammation

Links Between Gut Dysbiosis and Neurotransmitter Disturbance in Chronic Restraint Stress-Induced Depressive Behaviours: the Role of Inflammation

Authors - Hai‑long Yang
pigWB,IHC

Food Addit Contam Part A Chem Anal

FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.

Authors - Quancheng Liu
humanIHC

J Clin Exp Hematop

Role of TGF-beta1 and TNF-alpha1 produced by neoplastic cells in the pathogenesis of fibrosis in patients with hematologic neoplasms

Authors - Haruko Takizawa
humanWB

Front Immunol

PTPRC promoted CD8+ T cell mediated tumor immunity and drug sensitivity in breast cancer: based on pan-cancer analysis and artificial intelligence modeling of immunogenic cell death-based drug sensitivity stratification

Authors - Pengping Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alex (Verified Customer) (10-11-2019)

IL-2 Staining is dirty in mouse colon, attempted various dilutions and retrieval methods none of which worked.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Colon
IL-2 Antibody Immunohistochemistry, validation (1:2000 dilution) in Mouse Colon (Cat no:60306-1-Ig)