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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IL-2 Monoclonal antibody

IL-2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F11C5

Cat no : CL488-60306

Synonyms

Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated IL-2 Monoclonal anti (CL488-60306)

    IF Staining of human tonsillitis using CL488-60306

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 IL-2 antibody (CL488-60306, Clone: 2F11C5 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated IL-2 Monoclonal anti (CL488-60306)

    IF Staining of human tonsillitis using CL488-60306

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 IL-2 antibody (CL488-60306, Clone: 2F11C5 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60306 targets IL-2 in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-2 fusion protein Ag10199 相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight 153 aa, 18 kDa
GenBank accession numberBC070338
Gene symbol IL2
Gene ID (NCBI) 3558
RRIDAB_2919247
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IL-2 antibody CL488-60306Download protocol
Standard Protocols
Click here to view our Standard Protocols